Composite

Part:BBa_K769011:Design

Designed by: Mayumi Nakamura   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)


Promoter(Pbad)-RBS-lux operon-DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1680
    Illegal NheI site found at 5955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6417
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3767
    Illegal XhoI site found at 4275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1965
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1859
    Illegal BsaI.rc site found at 2541
    Illegal SapI site found at 961


Design Notes

bioluminescence


Source

Photobacterium kishitanii strain NCMB 844 lux-rib operon

References